复方中药对腹水综合征肉鸡肝组织α-SMA、I型和III型胶原蛋白含量的作用

doi:10.11843/j.issn.0366-6964.2016.11.020

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (11): 2310-2317.doi: 10.11843/j.issn.0366-6964.2016.11.020

复方中药对腹水综合征肉鸡肝组织α-SMA、I型和III型胶原蛋白含量的作用

韩烨晨¹，张娟¹，王俊武¹，员世宇¹，吴海萍¹，温爽¹，赵冬冬¹，王卓¹，苏晓菲²，朱昱波³，程佳¹，孙耀贵¹，王文魁¹，李宏全¹，段智变^1*

（1.山西农业大学动物科技学院，太谷 030801；2.山西省畜牧兽医学校，太原 030024；3.河南科技学院动物科技学院，新乡 453003）

收稿日期:2016-06-30 出版日期:2016-11-23 发布日期:2016-11-23
通讯作者: 段智变，女，山西祁县人，博士，教授，博士生导师，主要从事中兽医药对动物血管病变及脾胃病证研究，E-mail：duanzhibian2008@aliyun.com
作者简介:韩烨晨(1992-)，女，黑龙江省双鸭山人，硕士生，主要从事中药防治动物血管病变研究，E-mail：hanyechen1992@163.com
基金资助:
山西省科技攻关项目（20140311022-1）；山西农业大学横向科技项目（2013HX28）

The Effect of the Compound Recipe of the Traditional Chinese Medicine on Collagen in Liver of Broilers with Ascites Syndrome

HAN Ye-chen¹, ZHANG Juan¹, WANG Jun-wu¹, YUN Shi-yu¹, WU Hai-ping¹, WEN Shuang¹, ZHAO Dong-dong¹, WANG Zhuo¹, SU Xiao-fei², ZHU Yu-bo³, CHENG Jia¹, SUN Yao-gui¹, WANG Wen-kui¹, LI Hong-quan¹, DUAN Zhi-bian^1*

(1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China; 2. Shanxi Husbandry and Veterinary School, Taiyuan 030024, China; 3. College of Animal Scinece, Henan Institute of Science and Technology, Xinxiang 453003, China)

Received:2016-06-30 Online:2016-11-23 Published:2016-11-23

摘要/Abstract

摘要：

本试验旨在研究腹水综合征（AS）肉鸡肝组织α-平滑肌肌动蛋白（α-SMA）、I型胶原和III型胶原蛋白变化及复方中药的防治作用。将309羽7日龄罗斯肉鸡随机分为6组，空白组正常饲养，其余5组饲养环境为9~11 ℃、高能饲料和高钠饮水，模型组不给予任何药物，中药组（高、中、低剂量组）分别给予2、1、0.5 g•（kg•d）^－1复方中药，L-Arg组饲料中添加1% L-Arg。结果表明：15、25、35和45日龄，与空白组相比，模型组AS发病率、腹水心脏指数、心脏指数、肺脏指数、肝脏指数、肝组织α-SMA、I型胶原和III型胶原蛋白含量显著增加（P<0.01或P<0.05），脾脏指数显著降低（P<0.01或P<0.05）；与模型组相比，各剂量中药组和L-Arg组均能改善上述指标（P<0.01或P<0.05）。肉鸡腹水综合征肝组织α-SMA、I型胶原和III型胶原蛋白含量显著上升，参与AS病理发生发展；气血双补、行气利水的复方中药能有效防治AS，其机制与复方中药能显著降低AS肝组织α-SMA、I型胶原和III型胶原蛋白含量相关。

Abstract:

The objective of this study was to investigate the changes of α-smooth muscle actin (α-SMA), collagen type I and collagen type III in liver of broilers with ascites syndrome (AS) and the prevention and treatment of the compound recipe of the traditional Chinese medicine (TCM) on AS. A total of 309 7-day-old Ross broilers were randomly divided into six groups, group None was bred with normal diet, the rest were bred with high-energy diet derived from standard diet added with 3% lard and 4% fish meal and 0.12% Na+ in drinking water at 9-11 ℃ to induce AS, group Control was given no medicine, compound recipe of the TCM groups (High, Middle, Low) were respectively given a dose of 2, 1, 0.5 mL•kg^-1 of the TCM in drinking water, and group L-Arg with 1% L-arginine in diet. The results showed that at 15, 25, 35 and 45 days of age, compared with group None, the incidence of AS, ascites heart index, heart index, lung index, liver index, the levels of α-SMA, collagen type I and collagen type III in liver of broilers in group Control were increased significantly (P<0.01 or P<0.05), and spleen index in group Control was decreased significantly (P<0.01 or P<0.05). Compared with group Control, the items above were all improved in compound recipe of the traditional Chinese medicine groups and group L-Arg (P<0.01 or P<0.05). The study showed the increases of the levels of α-SMA, collagen type I and collagen type III in liver of broilers participated in the pathogenesis of AS. The studied compound recipe of the traditional Chinese medicine with the role of tonifying qi-blood and activating qi to excrete water can prevent and treat AS of broilers effectively, and the mechanism was related to the decreases of compound recipe of the traditional Chinese medicine on the levels of α-SMA, collagen type I and collagen type III in liver of broilers with AS.

中图分类号:

S853.33

韩烨晨，张娟，王俊武，员世宇，吴海萍，温爽，赵冬冬，王卓，苏晓菲，朱昱波，程佳，孙耀贵，王文魁，李宏全，段智变. 复方中药对腹水综合征肉鸡肝组织α-SMA、I型和III型胶原蛋白含量的作用[J]. 畜牧兽医学报, 2016, 47(11): 2310-2317.

HAN Ye-chen, ZHANG Juan, WANG Jun-wu, YUN Shi-yu, WU Hai-ping, WEN Shuang, ZHAO Dong-dong, WANG Zhuo, SU Xiao-fei, ZHU Yu-bo, CHENG Jia, SUN Yao-gui, WANG Wen-kui, LI Hong-quan, DUAN Zhi-bian. The Effect of the Compound Recipe of the Traditional Chinese Medicine on Collagen in Liver of Broilers with Ascites Syndrome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2310-2317.

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复方中药对腹水综合征肉鸡肝组织α-SMA、I型和III型胶原蛋白含量的作用

The Effect of the Compound Recipe of the Traditional Chinese Medicine on Collagen in Liver of Broilers with Ascites Syndrome

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